Fig. 8

P4HA1 regulates the TET2 -FBP1 pathway by modulating cellular α-KG level. A Cytoplasmic α-KG was measured in HUVECs infected with Ad-NC, Ad-P4HA1, Ad-shNC, or Ad-shP4HA1 (n = 3). B Western blotting analysis of protein levels of TET2, FBP1, and P4HA1 in HUVECs after treated with Ad-NC, Ad-P4HA1 alone, or Ad-P4HA1 and 1 mM octyl-α-KG for 24 h. Western blotting results were statistically analyzed (n = 3). C After HUVECs were treated with Ad-NC, Ad-P4HA1 alone, or Ad-P4HA1 and 1 mM octyl-α-KG for 24 h, a tube formation assay was conducted. Magnification: ×100, scale bar: 100 μm. Tube formation assay results were statistically analyzed (n = 3). D Schematic model showing that P4HA1 mediates glycolysis-induced angiogenesis by suppressing the TET2-FBP1 pathway via modulation of cellular α-KG levels. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. *p < 0.05. α-KG α-ketoglutarate, NC negative control