Fig. 7

TET2 mediates P4HA1-induced FBP1 downregulation. A Schematic diagram of the promoter region of FBP1 in the PGL3 plasmid. Circles in the promoter represent the binding sites of HNF4α. HUVECs were infected with Ad-NC and Ad-P4HA1 and then transfected with indicated plasmids. Firefly and Renilla luciferase activities were detected 48 h later. B Relative TET2 recruitment to the FBP1 promoter under P4HA1 overexpression and knockdown was determined using ChIP assays. C Western blotting analysis of protein level of HNF4α, TET2, and P4HA1 in HUVECs after infection with Ad-NC, Ad-P4HA1, Ad-shNC, and Ad-shP4HA1 for 24 h. Western blotting results were statically analyzed (n = 3). D Western blotting analysis of protein levels of TET2, P4HA1, and FBP1 in HUVECs after infection with Ad-NC, Ad-P4HA1 alone, or Ad-shP4HA1 and Ad-TET2 for 24 h. Western blotting results were statically analyzed (n = 3). E After HUVECs were infected with Ad-NC, Ad-P4HA1 alone, or Ad-P4HA1 and Ad-TET2 for 24 h, a tube formation assay was conducted. Magnification: ×100, scale bar: 100 μm. Tube formation assay results were statically analyzed (n = 3). Data in A were analyzed using the Student’s t-test. Data in B–E were analyzed by one-way ANOVA followed by Bonferroni post hoc test. *p < 0.05. NC negative control