Fig. 4

P4HA1 overexpression promotes glycolysis in HUVECs, and P4HA1 knockdown suppresses glycolysis in HUVECs. A GSVA based on RNA-seq data for two groups. B GSEA based on RNA-seq data for two groups. C RT-PCR analysis of the mRNA levels of PFKFB3, ALDOA, HK2, LDHA, GLUT1, and P4HA1 in HUVECs after infection with Ad-NC and Ad-P4HA1 for 24 h (n = 3). D RT-PCR analysis of the mRNA levels of PFKFB3, ALDOA, HK2, LDHA, GLUT1, and P4HA1 in HUVECs after infection with Ad-shNC and Ad-shP4HA1 for 24 h (n = 3). E, F HUVECs were infected with Ad-NC and Ad-P4HA1 for 24 h, E glucose uptake and F lactate production levels were determined (n = 3). G, H HUVECs were infected with Ad-shNC and Ad-shP4HA1 for 24 h. G Glucose uptake and H lactate production levels were determined (n = 3). I ECAR of HUVECs infected with Ad-NC and Ad-P4HA1 were measured by Seahorse XF96 Flux Analyzer. Glycolysis and glycolytic capability were statically analyzed (n = 3). J ECAR of HUVEC infected with Ad-shNC and Ad-shP4HA1 were measured by Seahorse XF96 Flux Analyzer (n = 3). Glycolysis and glycolytic capability were statically analyzed. Statistical differences between groups were analyzed using the Student’s t-test. *p < 0.05. NC negative control, ECAR extracellular acidification rate