Fig. 1

P4HA1 is upregulated in the HUVEC hypoxia model and ischemic muscle tissue. A Western blotting analysis of protein levels of HIF-1α, P4HA1, and ACTB in HUVECs under 0 h, 3 h, 6 h, and 12 h of hypoxia. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test (n = 3). *p < 0.05. B Western blotting analysis of protein levels of P4HA1 and ACTB in the gastrocnemius muscle of the HLI model and non-HLI mice. Data were analyzed by two-way ANOVA with Bonferroni post hoc test (n = 5). *p < 0.05 vs. the non-HLI group (3 d). ^#p < 0.05 vs. the non-HLI group (7 d). ^$p < 0.05 vs. the non-HLI group (14 d). C Hematoxylin-eosin staining and immunofluorescence staining of CD31 (green), P4HA1 (red), and DAPI (blue) in the gastrocnemius muscle tissue from the HLI model. Magnification: upper, 100×; lower, 400×. Scale bars: 100 μm. HLI hindlimb ischemia, non-HLI non-hindlimb ischemia, HE hematoxylin–eosin staining