Fig. 5

EXO1 inhibited lipid synthesis in prostate cancer. A–D GSEA analysis using the TCGA-PRAD project showed that EXO1 was associated with fatty acid transport and binding as well as protein-lipid complex formation. E–G Scatter plots were drawn using EXO1 expression as the horizontal coordinate and SREBP1, FASN, and SCD1 expression as the vertical coordinate. H Scatter plots with SREBP1 expression as the horizontal coordinate and FASN expression as the vertical coordinate. I Western blot detection of EXO1, SREBP1, and SREBP2 expression after knockdown of EXO1 in prostate cancer cells. J, K In prostate cancer cells, after the knockdown of EXO1, detect the expression of FASN and SCD1 by using qPCR. L–N In prostate cancer cells, after the knockdown of EXO1, lipid droplets were labeled using Oil Red O staining, and triglyceride and cholesterol levels were detected. O Overexpression of EXO1 in prostate cancer cells, and expression levels of EXO1, SREBP1, and SREBP2 were detected using Western Blot. P, Q After overexpression of EXO1, FASN, and SCD1 expression levels were detected in prostate cancer cells using qPCR. R–T After overexpression of EXO1 in prostate cancer cells, lipid droplets were labeled using Oil Red O staining, and triglyceride and cholesterol levels were detected