Fig. 3
From: COSMIC-based mutation database enhances identification efficiency of HLA-I immunopeptidome
Mutant peptides identified from HepG2 HLA-I immunopeptidome using HepG2 WES-based database. A. Kulbach leibier distance of different cluster numbers calculated by Gibbs cluster tool. B. MS-identified clustered to reveal the main binding motifs. C. NetMHCpan prediction of each MS-identified peptides assigned to different HLA alleles using HepG2 WES-based database. D. Distribution of NetMHCpan prediction strong, weak and non-binders according to the length of peptides. E. Lowest NetMHCpan predicted for binding which were rank and normalized to percentage value. F. MS2 spectra of a mutant HLA-I immunopeptide “SLFDASHML”. G. Elution rank distribution of mutant peptide and corresponding wild-type peptide in HLA-I immunopeptidome. H. Intensities distribution of mutant peptide and corresponding wild-type peptide in HLA-I immunopeptidome.