Fig. 2

HLA-I immunopeptides identified from HepG2 by IP-MS. A. Immunoblot showing HLA A/B protein levels in total cell lysate (TCL), flowthrough (FT) and elution. Table shows volumes used in western blotting analysis and corresponding grayscale value. B. HLA-A/B protein enrichment efficiency based on immunoblot result. C. Comparison of the matched MS spectra and identified peptides as well as proteins. D. Overlap of identified peptides for 3 biological replicates from HepG2 samples using Uniprot Human database. E. Distribution of Hyperscore of immunopeptides identified from 3 repeated HepG2 samples. F. Length distribution of with 8-14 a.a peptides identified from 3 repeated HepG2 samples. G. Overlap of 8-14 a.a identified peptides for three biological replicates from HepG2 cells using Uniprot Human database.