Fig. 6

ESM1 inhibits ANGPTL4 binding to integrin α5β1 and VE-cadherin, which represses HUVEC proliferation and migration to induce vascular permeability in the tumor microenvironment. A Co-IP showed that ESM1 inhibited ANGPTL4 binding to α5β1 and VE-cadherin in HUVECs cultured with HeyA8 and SKOV3 cells, respectively. The effect of vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1 on HUVECs cultured with Hey-A8 and SKOV3 cell lipid levels and proliferation ability via oil red O staining (B), MTT (C) and EdU (D) analysis. The migration ability was measured by wound healing (F) and Transwell assays (G) in HUVECs cultured with Hey-A8 and SKOV3 cells transfected with vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1. *P < 0.05, **P < 0.01, ***P < 0.001