Fig. 3

ANGPTL4 promotes OC growth and angiogenesis by activating the JAK2/STAT3 pathway in vivo. A Morphological observation and HE and IHC staining of xenografts in the NC-SKOV3, Vector-SKOV3, ANGPTL4 KD-SKOV3, ANGPTL4 KD-SKOV3 with Colivelin, NC-HeyA8, Vector-HeyA8, ANGPTL4 OE-HeyA8, and ANGPTL4 OE-HeyA8 with AG490 groups. B The tumor weight and volume and the IHC staining score of xenografts in the NC-SKOV3, Vector-SKOV3, ANGPTL4 KD-SKOV3, ANGPTL4 KD-SKOV3 with Colivelin, NC-HeyA8, Vector-HeyA8, ANGPTL4 OE-HeyA8, and ANGPTL4 OE-HeyA8 with AG490 groups. C The proliferation and tube formation of HUVECs cultured with the CM of HeyA8 cells were tested by EdU staining and tube formation assays. The chick chorioallantoic membrane assay measured the angiogenesis ability of HeyA8 CM in vivo. Angiogenesis ability of HeyA8 cells in a zebrafish model. The experimental groups were NC, Vector, ANGPTL4 OE and ANGPTL4 + AG490. D The proliferation and tube formation of HUVECs cultured with the CM of SKOV3 cells were tested by EdU staining and tube formation assays. The chick chorioallantoic membrane assay measured the angiogenesis ability of SKOV3 CM in vivo. Angiogenesis ability of SKOV3 cells in the zebrafish model. The experimental groups were NC, Vector, ANGPTL4 KD and ANGPTL4 KD + Colivelin. *P < 0.05, **P < 0.01, ***P < 0.001