Fig. 2

The effects of the ANGPTL4/JAK2/STAT3 pathway on OC proliferation, migration and invasion. A The protein levels of ANGPTL4, p-JAK2, JAK2, p-STAT3, STAT3, Vimentin, PCNA and hypoxia-inducible factor alpha (HIF-α) were detected in the NC-SKOV3, Vector-SKOV3, ANGPTL4 KD-SKOV3, ANGPTL4 KD-SKOV3 with Colivelin, NC-HeyA8, Vector-HeyA8, ANGPTL4 OE-HeyA8, and ANGPTL4 OE-HeyA8 with AG490 groups by Western blotting. B A workflow for transfecting cells, collecting conditioned medium, and conducting analyses on OC cells and HUVECs. The effect of ANGPTL4, ANGPTL4 + AG490, ANGPTL4 KD, and ANGPTL4 KD + Colivelin on Hey-A8 and SKOV3 cell proliferation ability via MTT (C) and EdU (D) analysis. The migration and invasion abilities of ANGPTL4, ANGPTL4 + AG490, ANGPTL4 KD, and ANGPTL4 KD + Colivelin were measured by wound healing (E) and Transwell invasion assays (F) in Hey-A8 and SKOV3 cells, respectively. *P < 0.05, **P < 0.01, ***P < 0.001