Fig. 5

TIMELESS augments glycolysis via upregulation of key metabolic Molecules. A Correlation analysis between TIMELESS expression and SIRT1, HIF1A and MYC in HNSC tissues form TCGA database. B Correlation analysis between TIMELESS mRNA levels and SIRT1 mRNA levels in 33 OSCC tissues. C qRT-PCR analysis of the expression levels of TIMELESS and key glycolysis genes in OSCC cells. D Western blot analysis of the expression levels of TIMELESS and key glycolysis genes in OSCC cells. E IHC staining of TIMELESS, SIRT1, HK2, PKM2, LDHA and GLUT1 in nude mice tumors. F The transfection efficiency was examined with western blot. G The level of glucose uptake was examined. H Lactate production was examined. I Cell medium pH. J Oxygen consumption level of cell. Data shown were the mean ± S.E.M. from three independent experiments. *P < 0.05; **P < 0.01