Fig. 2
From: TIMELESS promotes reprogramming of glucose metabolism in oral squamous cell carcinoma
TIMELESS enhances OSCC cell survival in vitro. A TIMELESS mRNA expression in OSCC cell lines in CCLE database. B Expression level of TIMELESS in OSCC cell lines and a normal oral keratinocyte was analyzed by western blot. *P < 0.05; **P < 0.01. C The transfection effect was verified by qRT-PCR. D The transfection effect was verified by western blot. E Clonogenic colony formation assays. F The growth rates were analyzed by MTS assay. G Representative images of EdU-positive cells (EdU staining, red). Scale bar, 50 μm. H The statistics results of cell cycle analysis. Data shown were the mean ± S.E.M. from three independent experiments. I Cell cycle‐related proteins, including cyclin D1, cyclin E1, CDK2, and CDK4 were detected by western blotting