Fig. 8
Requirement for EZH2 expression in differentiation of colonic mucosal CD4 and CD8 tissue resident memory T cells. Representative plots of cell-surface staining of CD69, CD103 of colonic IELs and LPLs from EZH2fl/flCD4cre mice and EZH2fl/fl mice with or without DSS induced chronic colitis. IELs were gated on CD3 + live cells. b, c Percentages of the CD69 + CD103 + cells of colonic IELs from EZH2fl/flCD4cre mice and EZH2fl/fl mice with or without DSS induced chronic colitis. Two independent experiments were performed (n ≥ 3), with similar results, and one experiment were used for statistic. d A animal model of BM chimeras. A BM chimeras were generated by mixing congenitally marked BM cells from Ezh2fl/flCD4-Cre mice (EZH2 knockdown; CD45.2, 40%) with BM cells from WT mice (CD45.1, 60%).Three mice at least per group were analyzed. e Representative plots of cell-surface staining of CD69,CD103 of colonic IELs and LPLs from BM chimeras mice in induced chronic colitis. IELs were gated on CD3 + live cells. f Percentages of the CD69 + CD103 + cells of colonic IELs and LPLs from BM chimeras mice in induced chronic colitis. n = 3