Fig. 7
Disrupted IELs’ Phenotype and increased inflammatory factors by rectal insulin instillation originated from upregulated colonic TRM. a Representative plots of staining of IFN-γ, Granzyme B, CD49, CD44, Klrg1, CD69 and CXCR6 of colonic IELs from KO(EZH2fl/flCD4cre)mice and WT(EZH2fl/fl)mice. n ≥ 3. IELs were gated on CD3 + live cells. b Representative plots of cell-surface staining of CD69, CD103 of colonic IELs in DSS group and DSS + insulin(12U/Kg) group. IELs were gated on CD3 + live cells. c Percentages of the CD69 + CD103 + cells of colonic IELs in DSS group and DSS + insulin (12U/Kg) group. Two independent experiments were performed with similar results (n ≥ 3), and one experiment were used for statistic. d Representative plots of cell-surface staining of CD69,CD103 of small intestine IELs from EZH2fl/flCD4cre mice and EZH2fl/fl mice. IELs were gated on CD3 + live cells. e Percentages of the CD69 + CD103 + cells of small intestine IELs from EZH2fl/flCD4cre mice and EZH2fl/fl mice. Two independent experiments were performed (n ≥ 3), with similar results, and one experiment were used for statistic