Fig. 6

EZH2 knockout alleviates colitis and exhibits opposite IELs’ phenotype to rectal insulin instillation. a–c. weight change, DAI score and colon length of EZH2^fl/flCD4^cre mice and EZH2^fl/fl mice in DSS induced chronic colitis. n ≥ 5. d.mRNA expression of IL-6 and TNF-ɑ of EZH2^fl/flCD4^cre mice and EZH2^fl/fl mice in DSS induced chronic colitis were analyzed by qPCR. n ≥ 5. e Representative plots of cell-surface staining of CD4,CD8α,CD8β and TCRγδof colonic IELs in DSS induced chronic colitis from EZH2^fl/flCD4^cre mice and EZH2^fl/fl mice. IELs were gated on CD3 + live cells. f Percentages of the indicated makers of colonic IELs in DSS induced chronic colitis from EZH2^fl/flCD4^cre mice and EZH2^fl/fl mice. n ≥ 3. The findings of three pooled independent experiments are shown. Two independent experiments were performed, with similar results, and one experiment were used for statistic. g Representative plots of Intracellular-staining of TNF-ɑ, IL-17 of colonic CD4 + and CD8αβ + IELs in DSS induced chronic colitis from EZH2^fl/flCD4^cre mice and EZH2^fl/fl mice.CD4 + and CD8αβ + IELs were gated on CD3 + live cells. Three independent experiments were performed, with similar results,and one experiment were used for statistic. h Percentages of TNF-ɑ, IL-17 in colonic CD4 + and CD8αβ + IELs in DSS induced chronic colitis from EZH2^fl/flCD4^cre mice and EZH2^fl/fl mice. n = 3