Fig. 5

Rectal insulin instillation enhances EZH2 expression and EZH2 inhibitor GSK126 reverses colitis exacerbation induced by rectal insulin instillation. a Differential Expression of EZH2 in human PBMC from T1D and T2D patients. Sequencing data GSE156035,GSE23561 were selected from GEO database. b mRNA expression of EZH2 in DSS group and DSS + insulin group were analyzed by qPCR. n ≥ 5. c Representative plots of Intracellular-staining of EZH2 in colonic IELs and LPLs from DSS group and DSS + insulin(12U/Kg) group.IELs and LPLs were gated on CD3 + live cells. d Percentages of high-EZH2 cells in colonic IELs and LPLs of DSS group and DSS + insulin(12U/Kg) group. n = 5. e A animal model schematic outline of Control group, DSS + insulin(12U/Kg) group and DSS + insulin(12U/Kg) + GSK126 group. Five mice at least per group were analyzed. f–h weight change, DAI score and colon length of Control group, DSS + insulin(12U/Kg) group and DSS + insulin(12U/Kg) + GSK126 group. n ≥ 5. i mRNA expression of IL-6 and TNF-ɑ in Control group, DSS + insulin(12U/Kg) group and DSS + insulin(12U/Kg) + GSK126 group were analyzed by qPCR. n = 3. j representative histological sections of the colon from Control group, DSS + insulin(12U/Kg) group and DSS + insulin(12U/Kg) + GSK126 group. Sections were stained with hematoxylin and eosin