Fig. 4

Rectal insulin instillation may exacerbate colitis by disrupting IELs’ subgroup and promoting inflammatory factors. Representative plots of cell-surface staining of CD4, CD8α, CD8β and TCRγδ in colonic IELs from DSS group and DSS + insulin(12U/Kg) group. IELs were gated on CD3 + live cells. Percentages of the indicated makers in colonic IELs of DSS group and DSS + insulin(12U/Kg) group. n = 3. The findings of three pooled independent experiments are shown. Two independent experiments were performed, with similar results, and one experiment were used for statistic. c Representative plots of Intracellular-staining of TNF-ɑ, IL-17 in colonic CD4 + and CD8αβ + IELs from DSS group and DSS + insulin(12U/Kg) group.CD4 + and CD8αβ + IELs were gated on CD3 + live cells. d Percentages of TNF-ɑ, IL-17 in colonic CD4 + and CD8αβ + IELs of DSS group and DSS + insulin(12U/Kg) group. n = 3. The findings of three pooled independent experiments are shown. e Representative plots of foxp3, CD25 and Klrg1 in colonic CD4 + LPLs from DSS group and DSS + insulin(12U/Kg) group. LPLs were gated on CD3 + CD4 + live cells. f Percentages of foxp3, Klrg1 in colonic CD4 + LPLs of DSS group and DSS + insulin(12U/Kg) group. n = 3.The findings of three pooled independent experiments are shown