Fig. 6

Cucurbitacin E alleviated cartilage degeneration in DMM mice. A Immunohistochemical staining of cartilage from the different groups (normal, DMM, DMM + Cucurbitacin E): IL-1β, IL-6, MMP13, and Collagen II. B Quantitative analysis corresponding to each immunohistochemical image for IL-1β, COX-2, MMP13 and Collagen II in C57BL/6 model mice, as determined by ImageJ software. The significance of differences between groups is expressed as P values (***P < 0.001, **P < 0.01, *P < 0.05,^nsP > 0.05)