Fig. 3

The PI3K/Akt pathway is activated in osteoarthritis chondrocytes. A Volcano plots of differentially expressed genes in normal and osteoarthritic cartilage based on the GSE114007 and GSE117999 datasets obtained from the GEO database. B KEGG enrichment analysis of differentially expressed genes in osteoarthritic cartilage. C Immunohistochemical staining of the p-PI3K protein in normal and osteoarthritic cartilage. D Western blotting was used to examine the activation of PI3K in IL-1β-stimulated osteoarthritis chondrocytes. E ImageJ software was used to analyse the activation of the PI3K protein. The significance of differences between groups is expressed as P values (**P < 0.01)