Fig. 2

Cucurbitacin E decreased the high expression of MMP13 and increased the low expression of Collagen II in IL-1β-stimulated osteoarthritis chondrocytes. A Figure showing the expression of MMP13 and Collagen II in IL-1β-induced osteoarthritis chondrocytes treated with different concentrations of Cucurbitacin E (1, 5, 10 nM), as measured by Western blotting. B and C Quantitative analysis of MMP13 and Collagen II protein expression, respectively (n = 3). D and E The expression of MMP13 and Collagen II in IL-1β-induced osteoarthritis chondrocytes treated with different concentrations of Cucurbitacin E (1, 5, 10 nM) was measured by qPCR. Immunofluorescence images of chondrocytes under different treatment conditions (Ctrl, IL-1β, Cucurbitacin E) are shown in F and G. H Diagram showing safranin O staining of chondrocytes under different treatment conditions (Ctrl, IL-1β, CuE). The significance of differences between groups is expressed as P values (***P < 0.001, **P < 0.01, *P < 0.05,^nsP > 0.05)