Fig. 6

Knockdown of ACACA attenuates PCa progression and reverses circKIF18B_003-induced malignant phenotypes in PCa cell lines. A–D Colony formation, CCK8, wound healing, and Transwell invasion assays were performed in PC-3 cells treated with vector, circKIF18B_003, circKIF18B_003 + ND630, and circKIF18B_003 + siACACA. E Western blot assay was used to determine the ACACA and p-AMPK protein expression levels in PC-3 cells treated as indicated. β-actin was used as a loading control. One-way ANOVA test and Kruskal–Wallis test were used for the statistical analyses. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns: no significant difference