Fig. 3

circKIF18B_003 acts as a sponge of miR-370-3p. A Schematic illustration exhibiting the overlapping circKIF18B_003 target miRNAs predicted by StarBase, CircBank, and NIH_interactome databases. B Putative binding site of miR-370-ep in circKIF18B_003 was predicted using StarBase version 3.0. C circKIF18B_003 and corresponding negative control probes were used in circRIP assays in circKIF18B_003-overexpression PC-3 cells. D RIP assays were performed using an antibody against AGO2 on extracts from PC-3 and DU145 cells. E. circKIF18B_003 level in the streptavidin captured fractions from the PCa cell lysates after transfection with biotinylated miR-370-3p or negative control. F The luciferase activity of pLG3-circKIF18B_003 in HEK-293 T cells after co-transfection with miR-370-3p mimics. G circKIF18B_003 and miR-370-3p were evaluated in the indicated cell lines by FISH assay. ^***P < 0.001; ns, not significant