Fig. 2

Effects of circKIF18B_003 on PCa Cell Proliferation, Invasion, and Migration. A The relative expression levels of circKIF18B_003 in DU145 and PC-3 cell lines post-transfection. DU145 cells were transfected with two shRNAs targeting circKIF18B_003 (for knockdown), while PC-3 cells were transfected with a plasmid promoting circKIF18B_003 overexpression. The graph shows a marked decrease in circKIF18B_003 expression in the DU145 cell line and a significant increase in the PC-3 cell line. B Colony formation assay results comparing the effects of circKIF18B_003 downregulation and upregulation on PCa cell viability. Downregulation of circKIF18B_003 led to a decrease in colony formation (indicating reduced cell viability), while upregulation had the opposite effect. C KIF18B protein expression levels in DU145 and PC-3 cells after transfection, showing that changes in circKIF18B_003 levels did not influence KIF18B expression. D Results from the Transwell invasion assay, demonstrating the impact of circKIF18B_003 modulation on PCa cell invasion capabilities. CircKIF18B_003 knockdown reduced cell invasion, while overexpression led to increased cell invasion. E CCK-8 assay results, further demonstrating the impact of circKIF18B_003 modulation on PCa cell viability. Consistent with the colony formation assay, circKIF18B_003 downregulation led to decreased cell viability, while its upregulation promoted cell viability. F Results from the wound-healing migration assay, illustrating the influence of circKIF18B_003 on PCa cell migration. Reduced cell migration was observed following circKIF18B_003 knockdown, while overexpression resulted in enhanced cell migration. Unpaired student’s test, Mann–Whitney U test, Kruskal–Wallis test and one-way ANOVA test were used for the statistical analyses. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001