Fig. 7

Effects of secreted products derived from the siRNA-transfected MDCK cells on ECs proliferation. Conditioned medium (CM) was harvested after 24-incubation of the siRNA-transfected MDCK cells in serum-free medium. ECs proliferation was determined by incubating EA.hy926 cells in CM mixed (1:1) with complete medium for up to 72 h. The EA.hy926 cells were collected at 24-, 48-, and 72-h time-points and subjected to cell count. A Micrographs of EA.hy926 cells after incubation with CM at indicated time-points. B Total cell numbers were counted using a hemacytometer. C Scatter plots of flow cytometric analysis at indicated time-points. D Total cell number quantified by flow cytometry. The dots on top of each bar represent individual data points derived from three biological replicates in three independent experiments, whereas the error bar represents mean ± SD of each group. Only significant p values are labeled