Fig. 5

Single and double knockdowns of ARID1A and ACTB by siRNA in MDCK renal cells. MDCK cells were individually or dually transfected with siRNA specific to ARID1A (siARID1A) and ACTB (siACTB). Efficacy of the siRNA transfection to silence expression of ARID1A and/or ACTB was determined by semi-quantitative RT-PCR compared with transfection with the control siRNA (siControl). A–C Gel images of mRNA bands of ARID1A, ACTB and GAPGH, respectively. D–E Quantitative analysis of mRNA band intensities of ARID1A and ACTB, respectively, normalized to GADPH. F-H Immunoblotting of ARID1A, β-actin and GAPDH, respectively. I–J Quantitative analysis of protein band intensities of ARID1A and β-actin, respectively, normalized to GADPH. The dots on top of each bar represent individual data points derived from three biological replicates in three independent experiments, whereas the error bar represents mean ± SD of each group. Only significant p values are labeled. M = marker ladder