Fig. 6

Inhibition of ferroptosis alleviates HFpEF. A Representative left ventricular M-mode echocardiographic tracings. B Representative pulsed-wave Doppler tracings. C Representative tissue Doppler tracings. D–F Percentage of LVEF; ratio between mitral E wave and A wave; ratio between mitral E wave and e′ wave (E/e′); n = 5 mice per group. G Body weights of the two groups of mice after 7 weeks of rearing, n = 5 mice per group. H Running distances of the two groups of mice during the exercise tolerance test, n = 5 mice per group. I Mouse tail blood glucose at different time points in the intraperitoneal glucose tolerance test, n = 5 mice per group. *Indicates the comparison between the HFpEF+Veh and HFpEF+Fer-1 group; ^#indicates the comparison between the HFpEF+Veh and HFpEF+DFP group. J Ratio between wet and dry lung weight, n = 5 mice per group. K Ratio between heart weight and tibia length, n = 5 mice per group. L, M mRNA levels of Bnp and myocardial fibrosis-related genes (α-SMA, Fn1, Col1a2, Timp1) in myocardial tissues of mice in the two groups, n = 5 mice per group. N–P Representative images of WGA, MT and CD31-stained heart sections from the two groups of mice. Q WGA quantification of cardiomyocyte cross-sectional area, n = 5 mice per group (scale bar: 50 μm). R Percentage of fibrosis area in MT-stained heart sections, n = 5 mice per group (scale bar: 50 μm). S Myocardial capillary density, n = 5 mice per group (scale bar: 50 μm). Statistical significance was calculated by one-way or two-way ANOVA; *P < 0.05, **P < 0.01, and ***P < 0.001