Fig. 3

Validation of ferroptosis-associated hub DEGs expression in the hearts of HFpEF mice. A–T Control mice and HFpEF mice were both fed a normal diet (Control) or HFD+L-NAME (HFpEF) for 5 weeks. A Representative left ventricular M-mode echocardiographic tracings. B Percentage of LVEF, n = 5 mice per group. C, D Representative pulsed-wave and tissue Doppler tracings, n = 5 mice per group. D–F Percentage of ratio between mitral E wave and A wave; ratio between mitral E wave and e′ wave (E/e′); n = 5 mice per group. G Body weights of the two groups of mice after 5 weeks of rearing, n = 5 mice per group. H Running distances of the two groups of mice during the exercise tolerance test, n = 5 mice per group. I Mouse tail blood glucose at different time points in the intraperitoneal glucose tolerance test, n = 5 mice per group. J Ratio between wet and dry lung weight, n = 5 mice per group. K Ratio between heart weight and tibia length, n = 5 mice per group. L, M mRNA levels of Bnp and myocardial fibrosis-related genes (α-SMA, Fn1, Col1a2, Timp1) in myocardial tissues of mice in the two groups, n = 5 mice per group. N–P Representative images of WGA, MT and CD31-stained heart sections from control and HFpEF mice. Q WGA quantification of cardiomyocyte cross-sectional area, n = 5 mice per group (scale bar: 50 μm). R Percentage of fibrosis area in MT-stained heart sections, n = 5 mice per group (scale bar: 50 μm). S Myocardial capillary density, n = 5 mice per group (scale bar: 50 μm). T Ferroptosis-associated hub DEG mRNA expression in control and HFpEF mice. Statistical significance was calculated by Student’s t test; *P < 0.05, **P < 0.01, and ***P < 0.001