Fig. 5

The effects of MEX3C suppression on LUAD cells could be reversed by inhibiting the expression of RUNX3. A, B Colony formation was evaluated for A549 and H1299 cells treated with si-MEX3C and/or si-RUNX3. C–E Representative images showing the results of Transwell cell migration and invasion assays of A549 and H1299 cells treated with si-MEX3C and/or si-RUNX3, scale bar, 50 μm. F Detection of proliferation in A549 and H1299 cells using an EdU assay, scale bar, 50 μm. G Flow cytometry assay to detect the cell apoptosis of A549 and H1299 cells treated with si-MEX3C and/or si-RUNX3. H Apoptosis and EMT-related protein expression were detected by western blot analysis. I Expression of Cleaved-Caspase-3 in A549/H1299 cells as analyzed by immunofluorescence assay, scale bar, 20 μm. Bars represent mean ± SD from 3 independent experiments. **P < 0.01