Fig. 3

Knockdown of MEX3C promotes apoptosis and inhibits proliferation in LUAD cells. A Colony formation was used to evaluated proliferation for A549 and H1299 cells with or without MEX3C knockdown. B Proliferation of A549 and H1299 cells was detected using an Edu assay, scale bar, 50 μm. C TUNEL staining was used to determine the potential impact of MEX3C knockdown on apoptosis in A549 and H1299 cells, scale bar, 50 μm. D Flow cytometry analysis was performed to measure the percentage of apoptotic cells in A549 and H1299 cells after indicated treatment. E The expression levels of apoptosis-related proteins (Bcl-2, Bax, and Cleaved-Caspase-3) in MEX3C-silencing A549 and H1299 cells were detected by western blot. F Expression of Cleaved-Caspase-3 in A549/H1299 cells as analyzed by immunofluorescence assay, scale bar, 20 μm. Bars represent mean ± SD from 3 independent experiments. **P < 0.01