Fig. 2

Knockdown of MEX3C in LUAD cells suppresses migration and invasion in vitro. A MEX3C mRNA and protein expression in human LUAD cell lines (A549, H1650, H838, and H1299) and a normal human bronchial epithelial cell-line HBE were analyzed by qRT-PCR and Western blotting, respectively. B The transcription of MEX3C in A549 and H1299 cells transfected with si-NC lentivirus or si-MEX3C lentivirus for 48 h was detected by qRT-PCR and western blotting analysis. C Cell migration and invasion of A549 and H1299 cells treated with or without knockdown of MEX3C were evaluated by Transwell assay. D A wound healing assay was used to assess the cell migration ability of A549 and H1299 cells treated with si-NC or si-MEX3C for 24 h. E EMT-related proteins E-cadherin and N-cadherin expression were detected by western blotting analysis. F Knockdown E-cadherin in A549 and H1299 cells using siRNA, E-cadherin expression was detected by western blotting. G The knockdown of E-cadherin was able to reverse the effect of MEX3C suppression on the migration and invasion of LUAD cell lines. Bars represent mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01