Fig. 1

Cell stemness-related genes CD44 and Oct4 promote the formation of breast cancer spheroid. A mRNA expression levels of stemness-related markers (MYC, NANOG, OCT4 and SOX2) in SP1–SP4. B Protein contents of stemness-related markers (CD44, OCT4, EpCAM and Vimentin) in SP1–SP4. C The gene heatmap based on RNA-seq of four cell clusters. D Gene trend analysis based on RNA-seq of four cell clusters. E The gene heatmap based on proteomics of four cell clusters. F Gene trend analysis based on proteomics of four cell clusters