Fig. 5

Establishment and characterization of colorectal cancer organoids. A Light microscopic (left column) and HE staining of four lines of organoids (middle column) and the corresponding tumor tissue (right column). HE, Hematoxylin–eosin staining; BF, Bright field. Scale bar 100 μm. B Non-synonymous mutated genes detected in CRC-1 and paired tumor tissue (CRC-1-Tumor). MultiHits indicates multiple mutations occur in the gene