Fig. 6

METTL3 expression is high in glioma tissues and cells. A RNA pull-down assay was performed using the HOTAIRM1 probe to enrich METTL3. B qRT-PCR was used to examine HOTAIRM1 expression levels in U87 and U251 cells transfected with either si-NC or si-METTL3. C qRT-PCR was performed to measure the transcriptional stability of HOTAIRM1 under METTL3 silencing in U87 and U251 cells. GAPDH was used as an internal control. D qRT-PCR and E western blot techniques were utilized to measure METTL3 expression level in glioma cells. F qRT-PCR and G western blot techniques were utilized to evaluate METTL3 expression level in glioma tissues. H The expression level of METTL3 in the glioma tissue sample was measured using immunohistochemistry (Scale bar = 50 μm). Data are presented as the mean ± SD of three independent experiments (n = 3). **P < 0.01, ***P < 0.001