Fig. 5

HOTAIRM1 facilitates glioma cell malignancy and promotes VM formation via regulating IGFBP2. A qRT-PCR was used to detect IGFBP2 expression level in glioma cells transfected with overexpressing IGFBP2 plasmid, si-IGFBP2 or control. B CCK-8 assay evaluated glioma cell proliferation in indicated transfected groups. C–D Transwell assay detected glioma cell migration and invasion capacity in indicated transfected groups (Scale bar = 50 μm). E Tube-formation assay was used to assess VM capacity in indicated transfected groups (Scale bar = 100 μm). Data are presented as the mean ± SD of three independent experiments (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001