Fig. 4

HOTAIRM1 interacts with IGFBP2. A The top five RNAs with binding sites to HOTAIRM1 were screened using the Starbase3.0 online tool. B Luciferase assay was used to detect the interaction of five candidate RNAs with HOTAIRM1. C Luciferase assay was used to detect the interaction of IGFBP2 and HOTAIRM1. D RNA pull-down assay confirmed the interaction relationship between IGFBP2 and HOTAIRM1. E qRT-PCR detected the expression of IGFBP2 in glioma cells after silencing HOTAIRM1. F qRT-PCR and G western blotting techniques were utilized to measure the IGFBP2 gene and protein expression levels in glioma tissues. Data are presented as the mean ± SD of three independent experiments (n = 3). ***P < 0.001