Fig. 2

Principal component analysis (PCA) plots and a bar graph of significant differentially expressed genes (DEGs) in human lung endothelial and epithelial cells after simulated ischemia–reperfusion (IR) or ex vivo lung perfusion (EVLP). A. PCA plot shows separation between epithelial and endothelial cell samples. B. PCA plots show three distinct clusters of CIT (red), D10 (green), and Steen (blue) samples. C. Compared with cells undergone CIT 18 h, endothelial cells had higher numbers of significant DEGs compared to those of epithelial cells in both IR and EVLP models. Statistical cut-off for significant DEGs: FDR-adjusted p-value < 0.05 and |log2 fold change|> 0.5