Fig. 3

The interaction network between APOE⁺ macrophages and iCAFs. A Cell–cell communications between main nine cell types by Cell chat analysis. B tSNE showing the composition of iCAFs. C Proportion of 5 major cell types showing in bar plots in different samples. D The Kaplan–Meier overall survival curves of HCC patients stratified by iCAFs infiltration. E, F Scatter plots showing the correlation between the infiltration of APOE⁺ macrophages and iCAFs in Zhongshan cohort and TCGA cohort. G The Kaplan–Meier overall survival curves of HCC patients stratified by the infiltration of both APOE⁺ macrophages and iCAFs. H Representative IF staining of human HCC tissue with MVI. PDGFRA (green), APOE (blue), CD68 (gold). Bar, 50 μm. I tSNE plot showing expression levels of SPP1 and CD44 in HCC samples. J The comparison of expression level of SPP1 and CD44 in MVI⁻ HCC and MVI⁺ HCC