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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: The HIFIA/LINC02913/IGF1R axis promotes the cell function of adipose-derived mesenchymal stem cells under hypoxia via activating the PI3K/AKT pathway

Fig. 2

LINC02913 expression affected ADSC cell phenotype under hypoxia and normoxia. A LINC02913 expression in ADSCs cells treated with different oxygen extensions was detected using qRT-PCR; B the transfection efficiency of LINC02913 overexpression plasmid (LINC02913 OE) was verified using qRT-PCR; C the changes in cell viability after LINC02913 overexpression under hypoxia and normoxic conditions were detected using CCK-8 assay; D the changes in the adipogenic ability of cells after LINC02913 overexpression under hypoxia and normoxic conditions were detected using oil red O staining; E after the induction for endothelial differentiation of ADSCs, cell tube formation after LINC02913 overexpression under hypoxia and normoxic conditions were detected using the tube formation assay; F the expression of endothelial cell marker CD31 was detected using IF staining after LINC02913 overexpression under hypoxia and normoxic conditions; G the level of endothelial cell markers (CD133, CD31, VEGFA, and eNOS) after LINC02913 overexpression under hypoxia and normoxic conditions was detected using western blot. **p < 0.01 compared to Nypoxia + vector group; ##p < 0.01, compared with Hypoxia+vector group

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