Fig. 8

ALKBH5 regulates lipid metabolism of pNENs by modulating PI3K/Akt/mTOR signaling pathway. A KEGG enrichment barplot based on ALKBH5 knockdown and scramble RNA-seq data. B, C Expression levels of PI3K/Akt/mTOR signaling pathway related proteins as determined by western blotting after ALKBH5 knockdown and over-expression in pNENs cells. D Expression levels of PI3K/Akt/mTOR signaling pathway related proteins as determined by western blotting in ALKBH5 over-expression cells with or without FABP5 knockdown. E, F LDs (lipid droplets) were detected using Nile red in QGP-1 cells from the indicated groups, along with the results of relative fluorescence intensity, magnification: ×200. G–I The cellular content of free fatty acids (FFA), triglycerides, and total cholesterol were detected in QGP-1 cells from the indicated groups. *p < 0.01, **p < 0.01, ***p < 0.001