Fig. 4

FABP5 is a functionally important target gene of ALKBH5. A Differentially expressed genes between ALKBH5 knockdown and control groups in QGP-1 cells as determined by RNA-sequencing. B Peak profiles in m6A modification after ALKBH5 knockdown in QGP-1 cells as shown by MeRIP-sequencing. C The m6A consensus motif is present in QGP-1 cells. D Distribution of m6A peaks across the length of mRNAs in QGP-1 cells with or without ALKBH5 knockdown. E The volcano plot showed the distribution of genes both differential (up or down) methylation level and differential (up or down) gene expression level in ALKBH5 knockdown and control groups. F Venn diagram showed the down-regulated genes, genes with elevated m6A methylation levels, and lipid-associated genes after ALKBH5 knockdown. G, H The validation of four candidate genes was verified by qRT-PCR in QGP-1 cells. I The protein expression of FABP5 in cells with ALKBH5 knockdown. J The relative abundance of m6A sites along FABP5 mRNA in QGP-1 cells with or without ALKBH5 knockdown, as detected by MeRIP-seq. *p < 0.01, **p < 0.01, ***p < 0.001