Fig. 3

ALKBH5 regulates lipid metabolism. A Enrichment analysis of KEEG and GO signal pathways. B Individual GSEA plots of fatty acid elongation, glycerolipid metabolism, and biosynthesis of unsaturated acid pathway in RNA-seq data from QGP-1 cells with ALKBH5 knockdown. C, D LDs (lipid droplets) were detected using Nile red in indicated cells with ALKBH5 over-expression, magnification: ×200, along with the results of relative fluorescence intensity. E–G The relative amounts of free fatty acids, triglycerides, and cholesterol were measured in cells with ALKBH5 over-expression. H, I The mRNA expression of dysregulation of genes involved in fatty acid synthesis (ACSL1, ACSL3, FABP5, SCD1, ACACA, MLYCD), fatty acid uptake (CD36), cholesterol biosynthesis (SREBF2 and HMGCR) in QGP-1 cells with ALKBH5 knockdown and over-expression. *p < 0.01, **p < 0.01, ***p < 0.001