Fig. 2
Knockdown of NR3C1 inhibits the proliferation of ccRCC. The effect of NR3C1 knockdown on the proliferation of ACHN A and 786-O cells B were assessed using CCK-8 assays. The concentrations of GC were: 100 nM, 400 nM, 800 nM, 1200 nM and 2000 nM. The effect of NR3C1 knockdown on the proliferation of ACHN C and 786-O cells D were detected using EdU assays. (GC = 1200 nM; scale bar = 100 μm). E The effect of NR3C1 knockdown on the colony formation ability of ccRCC cells. (GC = 1200 nM). Bar graphs represent the results of EdU F and colony formation assays G for both ccRCC cells. H Schematic overview for I–L. I, J Images of subcutaneous tumor formation in nude mice at 7 weeks after injection of 2×105 ACHN cells, with or without NR3C1 knockdown. Tumor volumes (K) and tumor weights (L) were measured for each group (n = 3). Data presented as mean ± SD with three replicates. Ns, no significant difference; *P< 0.05; **P<0.01; ***P< 0.001. GC, glucocorticoids