Fig. 6

Gene expression in KS samples and cell culture assays. A Total KSHV transcript per million (TPM) values versus STC1 and FLT4 TPM values were plotted. Spearman correlation analysis was performed for KS tumor samples. B Human dermal lymphatic endothelial primary cells (LEC) were infected with KSHV and RNA expression was analyzed 4 to 72 h post-infection using qPCR. C KSHV-infected iSLK cells were lytically induced and RNA was harvested 8 to 72 h after induction. D, E Viral transcripts (immediate early, IE; early, E; delayed-early, DE; late, L) were measured using genomic standard curves. F iSLK cells without KSHV infection were treated with doxycycline (Dox) to induce RTA expression. G Control SLK cells lacking inducible RTA were treated with combinations of Dox and sodium butyrate (NaB)