Fig. 6

miR-607 is a novel EGFR regulator and upregulated by cantharidin. A Schematic diagram of the EGFR-targeted miRNA screening. B mRNA level of miR-607 in wild-type and cantharidin-treated MDA-MB-231 and MDA-MB-468 cells. C mRNA level of miR-607 in wild-type, AG1478-treated and EGFR-knockdown MDA-MB-231 and MDA-MB-468 cells. D The expression of miR-607 was significantly lower in 81 TNBC tumor tissues than in 76 normal breast tissues in the TCGA database. E Analysis of the activation of miR-607 in the mild-type and mutant binding sites of EGFR using luciferase reporters in HEK293 cells. mRNA level (F) and protein level (G) of EGFR in miR-607-NC or miR-607-Mimic transfected MDA-MB-231 and MDA-MB-468 cells. H Statistical bar graph of Fig. 6G. Effects of miR-607 on cell proliferation in MDA-MB-231 (I) and MDA-MB-468 (J) cells by transfected with miR-607-NC, miR-607-Mimic or miR-607-Inhibitor. K Effects of miR-607 on the colony formation of MDA-MB-231 and MDA-MB-468 cells by transfected with miR-607-NC, miR-607-Mimic or miR-607-Inhibitor. L Effects of miR-607 on CCCP-induced cell apoptosis in MDA-MB-231 and MDA-MB-468 cells by transfected with miR-607-NC, miR-607-Mimic or miR-607-Inhibitor. Data were expressed as mean ± SEM (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001