Fig. 5

Cantharidin blocks MAPK/ERK signaling and triggers Caspase cascade. A Protein expression of p-MEK, MEK, p-ERK and ERK in MDA-MB-231 cells at indicated treatment. B Protein expression of p-MEK, MEK, p-ERK and ERK in MDA-MB-468 cells at indicated treatment. Immunofluorescence analysis of p-ERK protein in MDA-MB-231 (C) and MDA-MB-468 (D) cells treated with cantharidin, p-ERK (red), DAPI (blue) staining, FITC-actin (green) staining and merged images indicate the nuclear translocation and expression of p-ERK, the scale bar represents 20 μm. E Protein expression of p53, Bax, Bcl-2 and Mcl-1 in MDA-MB-231 cells at indicated treatment. F Protein expression of p53, Bax, Bcl-2 and Mcl-1 in MDA-MB-468 cells at indicated treatment. G Protein expression of APAF-1, Caspase9, Cle-casp9, AIF, Caspase3, Cle-casp3, PARP and Cle-PARP in MDA-MB-231 cells at indicated treatment. H Protein expression of APAF-1, Caspase9, Cle-casp9, AIF, Caspase3, Cle-casp3, PARP and Cle-PARP in MDA-MB-468 cells at indicated treatment