Fig. 3

Cantharidin restrains EGFR phosphorylation. A Protein expression and quantification of p-EGFR and EGFR in MDA-MB-231 cells at indicated treatment (cantharidin, gefeitinib, afatinib). B mRNA level of EGFR in MDA-MB-231 cells at indicated treatment. C Protein expression and quantification of p-EGFR and EGFR in MDA-MB-468 cells at indicated treatment. D mRNA level of EGFR in MDA-MB-468 cells at indicated treatment. E Protein expression and quantification of p-EGFR and EGFR in MDA-MB-231 and MDA-MB-468 cells. The cells were treated with cantharidin or pretreated with EGFR inhibitor AG1478 for 24 h. F Protein expression and quantification of p-EGFR and EGFR in MDA-MB-231 and MDA-MB-468 cells. The cells were stimulated by EGF cytokines at different times (10, 30, 60, 90 min). G Protein expression and quantification of p-EGFR and EGFR in MDA-MB-231 and MDA-MB-468 cells. The cells were treated with cantharidin or pretreated with EGF in MDA-MB-231 (10 min) and MDA-MB-468 (30 min) cells. Data were expressed as mean ± SEM (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001