Fig.5

UVR-induced immune cell infiltration during the formation of cSCC. A Immunochemical image of CD3; B Immunochemical image of CD4; C Immunochemical image of CD8; D Statistics of CD3 positive cells. After 12 weeks of UVR, the number of T cell increased and again grew significantly when the AK lesion developed into cSCC; E Statistics of CD4 positive cells. The CD4⁺T cells increased rapidly in photoaging skin and almost kept the quantity until AK occurred. When AK developed into cSCC, it rose significantly; F Statistics of CD8 positive cells in mice skin. The infiltration of CD8⁺T cells reduced in photoaging skin. When AK occurred, CD8⁺T cells sharply increased, especially in the epidermis; G Statistics of CD8 positive cells in epidermis; H The trend lines of CD8⁺T cell, CD4⁺T cell, CD3⁺T cell, and LVs value. Magnification of microscopic image: 100 × . *, p < 0.05 and **, p < 0.01 indicated a significant difference between groups; ns, no significant difference among groups