Fig.3

The dynamic changes of lymphatic density during the formation of cSCC. A OCT images of mice. B Histopathological results of mice. HE staining showed the thickness of the epidermis increased, and the dermis became thin after chronic UVR. C Image of LVs and D BVs immunochemistry staining in the dermis. E and F showed that the statistics of the epidermis and dermis thickness. Statistics of LVs G and BVs I density per area in immunochemistry staining. After eight weeks of UVR, the density of LV was at the lowest point. When AK lesions appeared, LV slightly out-numbered healthy skin. The lymphatic density in the dermis increased sharply when normal skin progressed into cSCC. The density of BVs increased rapidly when normal tissue progressed into photoaging, and AK lesions moved into cSCC. In immunochemistry staining, statistics of LVs H and BVs J diameter per area. Magnification of microscopic image: 100 × . *, p < 0.05 and **, p < 0.01 indicated a significant difference between groups; ns, no significant difference among groups