Fig.1

The relevance between lymphatic-centered immune microenvironments regulated by YAP1/VEGFC signal pathway and HNSCC patient cumulative survival time. A The heat map of VEGFC gene expression in HNSCC and healthy tissues. In HNSCC, the VEGFC gene showed an overexpression. P < 0.05; B The survival time relevance of LYVE1 gene expression analysis in cSCC was used by the exploration module (Gene_Outcome); C Image of LVs (LYVE1 with brown color, a particular marker of LVs) and BVs (CD31 with brown color, a unique feature of BVs) of human skin immunochemistry staining in the dermis. Magnification of microscopic image: 100 × . Statistics of the density of human LVs D and BVs E per area in immunochemistry staining. F The CD4⁺T cell and G CD8⁺T cell relevance of VEGFC gene expression in cSCC. The CD8⁺T cell relevance of VEGFC has a positive correlation. The CD4⁺T cell relevance of VEGFC has a negative correlation. P < 0.05; H The cumulative survival time relevance of CD8A⁺T cells and I CD8B⁺T cell infiltration in cSCC; J The association of VEGFC and YAP1 gene expression in HNSCC. They have a positive correlation. P < 0.05; When the YAP1 gene, endothelial cell K, immune cells L and M were in different expression or infiltration levels, respectively, the cumulative survival time of HNSCC patients differed. *, p < 0.05 and **, p < 0.01 indicated a significant difference between groups; ns, no significant difference among groups