Fig. 8

Correlations between DARS2 expression and glycolysis in LUAD. A Flow cytometry results of glucose uptake in LUAD cells determined by 2-NBDG staining. B Glucose uptake capacity was calculated using average fluorescence intensity. C Determination of lactate production in different groups of LUAD cells. D The lollipop diagram shows the correlation between DARS2 and the expression of glycolysis related genes. E Differential expression analysis of glycolysis related genes between DARS2 high and low expression groups. F Determination of the expression level of 11 glycolytic genes in different groups of LUAD cells. G The Venn diagram shows the positive genes that match the above three studies. ns means not significant; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001